Smai cut site

Enzymes and Inhibitors.

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Smai cut site

Hi all, 1 Has anyone used smaI as a RE to cut a vector for blunt end cloning? So my situation now is I'm trying to cut a plasmid bp with smaI. I over digest overnight at room temp. Hope you guys can shed some light SmaI is not the best RE out there, but I understand that sometimes you'd have to use it since it's your only choice. I'd definately phosphatase my plasmid in order to prevent self ligation. I recently used Antarctic Phosphatase and it workes also very good. Good luck!!. Sma I has an isoschizomer which may be a better enzyme. If you still need the blunt end, you can just do a fill-in or chew back the overhang.

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Smai cut site

Learn more. We are excited to announce that all reaction buffers are now BSA-free. Find more details at www. Web pricing is applicable only to orders placed online at www. Notes SmaI is an isoschizomer of XmaI. Blocked by CpG methylation. Is SmaI affected by methylation? How does SmaI differ from its isoschizomer, XmaI? What is the molecular weight of SmaI?

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Enzymes and Inhibitors. Restriction Enzymes. Catalog number: ER

We use these cookies to remember your settings and preferences. This field is required. Confirm New Password Passwords don't match. Please request another reset link. Preference Cookies We use these cookies to remember your settings and preferences. Log in with Your New Password. Resend Verification Email. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. SmaI is not the best RE out there, but I understand that sometimes you'd have to use it since it's your only choice. I'd definately phosphatase my plasmid in order to prevent self ligation. Pacific Asia Australia.