N benthamiana

Data are scarce about the influence of basic cultural conditions on growth patterns and overall performance of plants used as heterologous production hosts for protein pharmaceuticals. Higher plants are complex organisms with young, n benthamiana, mature, and senescing organs that show distinct metabolic backgrounds and differ in their ability to sustain foreign protein expression and accumulation. Here, we used the transient protein expression host Nicotiana benthamiana as a model to map the accumulation profile of influenza virus n benthamiana H1, a clinically promising vaccine antigen, n benthamiana, at the whole plant scale. Greenhouse-grown plants submitted to different light regimes, submitted to apical bud pruning, or treated with the axillary growth-promoting cytokinin 6-benzylaminopurine were n benthamiana with agrobacteria harboring a DNA sequence for H1 and allowed to express the viral antigen for 7 days in growth chamber under similar environmental conditions.

Federal government websites often end in. The site is secure. Nicotiana benthamiana is widely used as a model plant for dicotyledonous angiosperms. In fact, the strains used in research are highly susceptible to a wide range of viruses. Accordingly, these strains are subject to plant pathology and plant—microbe interactions.

N benthamiana

Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. An Author Correction to this article was published on 05 January To further improve its usefulness and versatility, we have produced high-quality chromosome-level genome assemblies, coupled with transcriptome, epigenome, microRNA and transposable element datasets, for the ubiquitously used LAB strain and a related wild accession, QLD. In addition, single nucleotide polymorphism maps have been produced for a further two laboratory strains and four wild accessions. Despite the loss of five chromosomes from the ancestral tetraploid, expansion of intergenic regions, widespread segmental allopolyploidy, advanced diploidization and evidence of recent bursts of Copia pseudovirus Copia mobility not seen in other Nicotiana genomes, the two subgenomes of N. LAB and QLD have many genetic, metabolic and phenotypic differences, including disparate RNA interference responses, but are highly interfertile and amenable to genome editing and both transient and stable transformation. Like most Solanaceae, it has a basic chromosome number of 12, with haploid DNA content ranging from 1. Section Suaveolentes nicely smelling includes N. The diploid ancestors of N. Chromosome numbers are indicated for each Suaveolentes species. Species highlighted by an asterisk are extant relatives of the putative parents of N.

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Nicotiana benthamiana , colloquially known as benth or benthi , is a species of Nicotiana indigenous to Australia. It is a close relative of tobacco. A synonym for this species is Nicotiana suaveolens var. This was transferred to Nicotiana benthamiana by Karel Domin in Bibliotheca Botanica , honoring the original author in the specific epithet. The plant was used by people of Australia as a stimulant, containing nicotine and other alkaloids , before the introduction of commercial tobacco N.

Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. An Author Correction to this article was published on 05 January To further improve its usefulness and versatility, we have produced high-quality chromosome-level genome assemblies, coupled with transcriptome, epigenome, microRNA and transposable element datasets, for the ubiquitously used LAB strain and a related wild accession, QLD. In addition, single nucleotide polymorphism maps have been produced for a further two laboratory strains and four wild accessions.

N benthamiana

Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. Nicotiana benthamiana is a workhorse for fundamental research and biotechnology. We assembled and annotated the allotetraploid chromosomal-level genomes and epigenomes of the laboratory strain and a wild strain of Nicotiana benthamiana , creating a reference genome for the genus Nicotiana. This is a preview of subscription content, access via your institution. Bally, J. The rise and rise of Nicotiana benthamiana : a plant for all reasons. A review of N.

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Narayan, R. The percentages of complete single copies and duplications were Liu, J. Dudchenko, O. Dorais, M. Genome Biol. Supplementary information Supplementary Information Supplementary Figs. Yang, S. Federal government websites often end in. The possible contribution of axillary leaves as source organs was assessed in parallel to underscore an eventual reliance of young, fast-growing P1 and P2 leaves on external metabolic resources from the S production units during H1 expression. On the other hand, the maternal lineage might be the ancestor of the Noctiflorae section; however, the details are less clear. Goulet, C. Effect of climate conditions and plant developmental stage on the stability of antibodies expressed in transgenic tobacco.

Nicotiana benthamiana is widely used as a model plant for dicotyledonous angiosperms. In fact, the strains used in research are highly susceptible to a wide range of viruses.

Not all scaffolds had gene-dense regions at both ends, and telomeric repeats were detected only in some scaffolds, indicating that they did not cover the entire region as complete chromosomes Supplementary Fig. Wikimedia Commons Wikispecies. Supplementing these high-confidence genes with those lost in the analysis but identified by Scallop gave 45, and 49, genes for LAB and QLD, respectively. The high-quality reads from whole-genome bisulfite sequencing samples were aligned to LAB and QLD genome assemblies using the default settings of the Bismark program v. Nicotiana benthamiana is one of the most widely distributed experimental plants. Feeding responses to solanaceous allelochemicals by larvae of the tobacco hornworm, Manduca sexta. Greenhouse-grown plants submitted to different light regimes, submitted to apical bud pruning, or treated with the axillary growth-promoting cytokinin 6-benzylaminopurine were vacuum-infiltrated with agrobacteria harboring a DNA sequence for H1 and allowed to express the viral antigen for 7 days in growth chamber under similar environmental conditions. The genome analysis of a section Noctiflorae , another progenitor of N. Although QLD has a greater genetic spectrum for metabolic and biotechnological engineering than LAB and similarly high transformation and gene editing efficiencies, its slower growth rate and lower yields of transiently expressed antibodies following vacuum agro-infiltration make LAB the preferred choice as a biofactory and research tool. The EDTA pipeline v. Tools Tools. PLoS One 8:e Relative contribution of P and S leaf production units to total biomass and H1 yield per plant following heterologous expression.