Drfive

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Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. Indeed, apoptosis and non-apoptotic signaling can be activated in clonal populations of cancer cells in response to treatment and, as a result, only a part of the initial cellular population dies while a fraction survives and develops resistance to TRAIL-induced apoptosis referred to as 'fractional survival'. Notably, the molecular characterization of the protein platforms streaming into tumoricidal versus tumor-promoting cascades that control fractional survival remained elusive.

Drfive

Your browser does not have JavaScript enabled and some parts of this website will not work without it. For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome. This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free production For more information see here. Our Abpromise guarantee covers the use of ab in the following tested applications. Lanes 1- 2: Merged signal red and green. Green - ab observed at 47 kDa. Loss of signal was observed when knockout cell line ab knockout cell lysate ab was used. Membrane was blocked for 1 hour at room temperature in 0. Confocal image showing cytoplasmic staining on HT cells. The nuclear counter stain is DAPI blue.

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Drfive

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We haven't added this to the BETA yet. The Abpromise guarantee Our Abpromise guarantee covers the use of ab in the following tested applications. Having a unscramble tool like ours under your belt will help you in ALL word scramble games! Abreviews Abreviews. Detects a band of approximately 48, 40 kDa predicted molecular weight: 48 kDa. TRAF2 sets a threshold for extrinsic apoptosis by tagging caspase-8 with a ubiquitin shutoff timer. Nat Rev Cancer ; 8 : — In the coming months Additional product types Supporting content Sign in to your account Purchase online. Mol Cell ; 56 : — At this time point, the complete subpopulation of apoptotic cells was detached allowing for its direct collection, while surviving cells were collected after treatment with trypsin. Additional cross-reactive bands were observed in the wild-type and knockout cells. Thank you for visiting nature. Following the discovery of 'cancer cell-selective' apoptosis by TRAIL, several strategies have been explored for cancer therapy.

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At this time point, the complete subpopulation of apoptotic cells was detached allowing for its direct collection, while surviving cells were collected after treatment with trypsin. Collectively, this study reveals the Janus faces of DR5, and the contributions of other death complex components in fractional survival that foster the generation of resistance. Science ; : — Altogether, these results suggest that the activity of initiator procaspase s represents a first node of divergence for the activation of individual TRAIL-induced non-apoptotic pathways. We thank Cathie Erb for technical advice. Collectively these results reveal that both DcR2-dependent and DcR2-independent mechanisms counterbalance cell death and supports that DR5 is indeed the key receptor mediating TRAIL-induced cell death in this cellular system. Search Search articles by subject, keyword or author. Read More. Subpopulations displaying low 1 and high 2; percentage is depicted levels of caspase-8 cleavage are indicated. VP and HG supervised the work, recruited financial support and edited the manuscript. Supplementary Figure 1 JPG kb.