Superscript iii one step rt pcr system

Federal government websites often end in. The site is secure. Real-time reverse transcription polymerase chain reaction RT-PCR is a commonly used technique to analyze gene expression. There has been little research conducted to test if SuperScript III quantitative one-step reverse transcription carried out in the same tube as PCR and two-step reverse transcription carried out in a separate reaction RT-PCR systems provide similar real-time results.

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Superscript iii one step rt pcr system

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View more versions of this product. Catalog No. The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product. By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Superscript iii one step rt pcr system

View more versions of this product. Catalog No. Due to product restrictions, please Sign In to purchase or view availability for this product. The amount of starting material can range from 0.

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Learn More. SuperScript III. Documents and Certificates. Reaction Format. Organic Compounds. Therefore, the same amount of starting RNA template was used for both one-step and two-step methods. This work was published in and since that time significant improvements in development of enzymes have been made in one-step reaction kits. Life Sciences Products. J Biomol Tech ; 15 — A concern for the one-step reaction is that it has been shown that the reaction efficiency for RT enzymes varies with RNA concentration.

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Tubing and Accessories. These products typically do not have pictures or detailed descriptions. Spill Control and Containment. For each run, a standard curve was generated with log of the RNA concentration on the X-axis and cycle threshold on the Y-axis. Unity Lab Services. In order to test one-step and two-step methods in various conditions, total RNA from two different tissue types were obtained human skeletal muscle and brain and three differently expressed genes were purposely chosen. Real-time reverse transcription polymerase chain reaction RT-PCR is a commonly used technique to analyze gene expression. Account Administration. Request New Item. Biochem Biophys Res Commun ; — Shipping and Billing Addresses.