Spectra viewer

The SpectraViewer tool enables you to visualize the spectral compatibility of fluorophores and fluorescent spectra viewer probes. Revvity Sites Globally Select your location. How to use the SpectraViewer If you're using a Revvity imaging or detection instrument, start by selecting the instrument from the Select Machine drop-down menu. The light source and filters for the chosen instrument will be displayed automatically, spectra viewer.

Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation. The continuous development of dyes with improved spectral profiles combined with breakthroughs in light sources, detection methods, and interference filters have paved the way to the adoption of multiplex analysis beyond the realm of flow cytometry.

Spectra viewer

Navigation Menu. AAT Bioquest. Cart 0. Sign In. Add a spectrum to begin. Compound Name. How to use this tool AAT Bioquest's interactive Spectrum Viewer is a powerful tool for analysis and comparison of the spectra of common compounds used in absorbance and fluorescence formats. The Spectrum Viewer is set to fluorescent mode by default. To use the viewer for absorbance dyes colorimetric format labels , click the left drop-down menu under "Current mode" and select "Absorbance". For technical assistance on using this web application, please contact websupport aatbio. To display the information about a specific compound, touch the displayed curve of that compound, and the associated properties will be displayed on the right-hand side of the page.

To search for an appropriate filter, use the right-side menu section to spectra viewer by part type, manufacturer, or instrument. Click "Disable Intensity Prediction" to return to the default visual display.

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Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation. The continuous development of dyes with improved spectral profiles combined with breakthroughs in light sources, detection methods, and interference filters have paved the way to the adoption of multiplex analysis beyond the realm of flow cytometry. Acquiring large amounts of relevant biological information for each sample has become paramount also in microscopy and imaging experiments. One of the most challenging aspects of multiplex fluorescence analysis is the selection of a combination of fluorochromes with different emission spectra. Spectral overlapping can, in fact, undermine the accuracy and validity of the experiment.

Spectra viewer

Navigation Menu. AAT Bioquest. Cart 0. Sign In.

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The filter will now appear on the display as a semitransparent vertical band in the color associated with its location on the spectrum. If the "Custom" row is selected, a popup box will appear and prompt the user to enter the center wavelength. If applicable, a list of similar compounds, as well as related categories, will be displayed as well. Spectral overlapping can, in fact, undermine the accuracy and validity of the experiment. Light sources are input in nm format and filters as - Next, select the fluorophores of interest from the Add Fluorophore drop-down menu. To search for an appropriate excitation source, use the right-side section to search by part type, manufacturer, or instrument. The user may move this excitation source by hovering over the representative vertical line until the cursor changes shape to a symmetrical horizontal arrow; once the cursor has changed shape, left-click and hold while dragging the line to a desired place along the horizontal axis. Trusted by leading Companies, Labs and Core Facilities worldwide. To select a known filter, select one or more options on the left menu. To predict the response of the fluorophores on the graph to an excitation laser, click "Predict Intensity" under the "Analyze" submenu. The Spectrum Viewer is set to fluorescent mode by default. Use Print to generate a printed version of your selections and the corresponding spectra.

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Pre-loaded light sources and laser sets for microscopy and flow cytometry applications. Compound Name. A menu will appear below the graph display with common generic filters displayed on the left. Add a spectrum to begin. Email Required. Note: If a filter is added to the graph, a new column will appear in the information table at the bottom of the page, labeled "Spillover" with the filter shown in parentheses. Cart 0 Sign In. To remove a filter from showing on the graph, deselect the check box to the right of the filter title. Click one or more choices. The Spectrum Viewer is set to fluorescent mode by default. To predict the response of the fluorophores on the graph to an excitation laser, click "Predict Intensity" under the "Analyze" submenu. To exit out of the menu, click the small "x" on the upper right corner of the gray bar at the top of the menu.