Invitrogen molecular probes

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Invitrogen molecular probes

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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented. Alexa Fluor dyes are among the most trusted fluorescent dyes available today. For stable signal generation in imaging and flow cytometry, Alexa Fluor dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

Invitrogen molecular probes

Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection. Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products. Dextrans are hydrophilic polysaccharides characterized by their moderate-to-high molecular weight, good water solubility, and low toxicity. They also generally exhibit low immunogeniticy. Furthermore, we use rigorous methods for removing as much unconjugated dye as practical, and then assay our dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants. Dextran Net Charge and Fixability We employ succinimidyl coupling of our dyes to the dextran molecule, which, in most cases, results in a neutral or anionic dextran. Some applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis. These dextrans have covalently bound lysine residues that permit dextran tracers to be conjugated to surrounding biomolecules by aldehyde-mediated fixation for subsequent detection by immunohistochemical and ultrastructural techniques.

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Laboratory Balances. Centrifuge Adapters. Volumetric Pipettes. Close Confirm My Choices. View All Molecular Biology. This information might be about you, your preferences or your device and is mostly used to make the site work as you expect it to. These cookies are necessary for the website to function and cannot be switched off in our systems. These cookies do not store any personally identifiable information. Centrifuge Buckets. Cell Culture Flasks.